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Reaction mixture II for each sample was collected as follows: 1160 l of 0.1-M Tris-HCl buffer with 0.34-mM EDTA (pH 8.5) were sequentially added to 108 l of each supernatant containing residual GSH, and then at the same intervals, 9 l of a solution of 5,5-dithiobis(2-nitrobenzoic acid) or Ellmans reagent (4 mg/ml of absolute methanol) was added to each sample (in the same sequence of samples)

Mitochondrial oxidative stress in Alzheimer's disease (AD) drives A accumulation and tau hyperphosphorylation, exacerbating neuronal damage (70)

Kornblau SM, Qiu YH, Graber G, Chen W, Melendez J, Konopleva M, Faderl S, Andreeff M , Borthakur G, Yao H, Coombes KR

Acta Physiol (oxf) 213(3):586594

Concomitantly, serine/threonine phosphatases and phosphotyrosine phosphatases are prone to oxidation-induced inactivation