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Immunofluorescence Cells were first seeded on sterile glass slides (Servicebio, #WG655) and cultured for 24 h
The eluate was loaded onto a HiLoad 26/60 Superdex 200 pg column with SDX buffer (50 mM HEPES [pH 7.5], 150 mM NaCl, 5% glycerol, 1 mM DTT, 1 mM MgCl 2 )
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Relative vascular permeability and vascularity across different regions of the rat nasal mucosa: implications for nasal physiology and drug delivery
