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For genotyping PCR, following primers were used: 5-GCCTGGTATTCACATTGGAA and 5-GAGTGGATAAGAGTGACCTG for the wild-type allele

Blood was collected from these mice at different time points, and plasma was processed and analyzed by LC/MS after a reduction and alkylation step under reducing conditions to capture total peptide (free peptide and peptide bound to plasma proteins by disulfide formation)

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Schedule urgent care within 24-48 hours for new foot drop, severe unresponsive pain, or rapidly worsening symptoms

10.1016/j.expneurol.2006.12.020 88 HaydenK