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As a primary screening, genomic DNAs from the tail of the pups were subjected to genomic PCR to identify animals with the transgene using the three primer pairs covering different parts of the construct (pair A: pCAGGS_FW2 : 5TAACCATGTTCATGCCTTCT3 and Mpst_Rv1 : 5TTTACTGAGCCAGGGATGT3, pair B: Mpst_Fw1 : 5ATCTACGACGACAGTGACCA3and globinA_Rv1 : 5GTCGAGGGATCTCCATAAGA3, and pair C: CAG_Fw4 : 5ACCTGGGTCGACATTGATTA3 and CAG_Rv2 : 5AACATGGTTAGCAGAGGCTC3)

Optimization of Collagenase Proteomics for Improved Mass Spectrometry Imaging Peptide Identification

A promising alternative is the metabolic engineering of microorganisms

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Its Olink Explore HT platform covers approximately 5,400 protein targets and is based on proximity extension assay (PEA) technology (Olink, 2024)