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In addition, when glutamate or NMDA was injected directly into crush injured sciatic nerves, ERK1/2 signaling was again observed in both myelinating and nonmyelinating Schwann cells [271]

The most recent improvement to the method for generating amber codon mutants was the development of the plasmid pEVOL (Young et al., 2010)

This is where certain gut-derived peptides, notably GLP-1, GLP-2, and VIP (Vasoactive Intestinal Peptide), show remarkable therapeutic promise

The fragments were integrated either into pSL4 (generating C-terminal 10xHis fusions) or into pDS94 (generating C-terminal GFP-10xHis fusions) (Plasmid list in Supplementary Table 4)