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Rational design of diaryl ether-based dual inhibitors targeting successive essential enzymes HadAB and InhA in Mycobacterium tuberculosis
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The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation
