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For a starting dose of 0.25 mg, you would draw about 7.5 units

doi: 10.1016/s0950-351x(98)80478-3

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Mutations of key residues (F93, L95, L65, V99), predicted from the structure to mediate channel-cholesterol interactions, ablated cholesterol potentiation in a heterologous expression system (Mathiharan et al., 2021)