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A major obstacle is the lack of validated surrogate biomarkers that can predict patient response, monitor therapeutic efficacy, or assess the safety of interventions targeting this axis (164, 168)

Basic Scientific Research Project of the Provincial Department of Education (Grant No

S., Dasarathy, S

Upon establishing the kinetics of shRNA-mediated knockdown of these proteins in H1299 cells, we determined the onset of protein depletion to be 48 h post-lentiviral infection (time-point 0) (Fig

Protein concentration GSH (g/mg prot)=yVRVVRVCpr =yCpr Sample weight GSH (g/g)=yVRV(VRVVSVW)= yW Cell amount GSH (g/104cell)=yVRV(VRVVSVN)= yN Solution volume GSH (g/mL)=2y N: Cell amount, 106