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The transfection complex was formed at a DNA:PEI ratio of 1:3 in OPTI-MEM (Life Technologies, Paisley, UK), with a 30 minute incubation at room temperature prior to addition to the cells

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Khalatbari-Soltani S, Tabibi H (2015) Inflammation and L-carnitine therapy in hemodialysis patients: a review

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Aspiration means pulling back slightly on the plunger after inserting, to check for a flash of blood (indicating youve hit a vessel) before injecting

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