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Then, the well plates were washed with 1 ml of 0.9% NaCl, and the cells were lysed with 1 ml of chloroform:MeOH:H 2 O (1:3:1) and centrifuged at 15,000 g for 5 min at 4 C

Many peptidesparticularly long-chain, cyclic, or hydrophobic moleculesare sensitive to: Mechanical stress Rapid temperature changes Oxidation Improper pH Surface adsorption Repeated freeze-thaw cycles Ignoring these factors may reduce peptide quality long before the experiment begins

S., Liu X., et al., Asparagine Endopeptidase Cleaves synuclein and Mediates Pathologic Activities in Parkinson's disease, Nature structural & molecular biology 24, no

All peptide stacks supplied by Tides Lab are intended strictly for laboratory research and analytical purposes and are not approved for human consumption

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