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They were seeded onto 6-well cell culture plates and transiently transfected with different GLP-2R constructs using Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA)

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Qsymia is approved for chronic, long-term use, which separates it from phentermine alone (limited to 12 weeks in most prescribing guidance)

This increase in fundic mucosal GLP-1 following RYGB but not VSG was consistent in diet-induced obese rats, where instead, VSG surgery induced a 50% increase in GLP-1+ cells in the antrum (Ribeiro-Parenti et al., 2021)