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[17] [18] Additional risk factors include: Female sex and pregnancy history : Women, particularly those who have been pregnant, have higher baseline gallstone risk Age over 40 years : Gallbladder disease incidence increases with age Obesity : Higher BMI correlates with increased gallstone prevalence Diabetes : Type 2 diabetes itself is associated with gallbladder dysfunction Rapid weight cycling : History of repeated weight loss and regain Certain ethnicities : Native American and Hispanic populations have higher gallstone rates [23] [24] Family history : Genetic predisposition to gallbladder disease Estrogen therapy : May increase cholesterol saturation in bile Patients with multiple risk factors should engage in thorough discussion with their healthcare provider about symptom awareness

Strengthening the reporting of observational studies in epidemiology using Mendelian randomization: the STROBE-MR statement

After consuming a carbohydrate-rich meal, glucose begins entering the bloodstream

The default is once daily, continuous

Primers for amplicon preparation are listed in Supplementary Table 5
