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P0 viral stock was produced by transfecting 5 g recombinant bacmids into Sf9 cells (2.5 mL, density of 1 million cells per mL) for 96 h incubation and then used to produce P1 and P2 baculovirus

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Back Lund, PK (2005) 'The discovery of glucagon-like peptide 1', Regulatory Peptides , 93-96

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