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Description
Influence how the body stores and burns energy, potentially raising calorie expenditure
B85502, Invitrogen) cultured in flat culture flasks were grown until confluent in Express Five SFM Medium (Gibco) supplemented with 200 mM l-glutamine (Sigma-Aldrich) and 1 Antibiotic-Antimycotic solution (Sigma-Aldrich) at 27 C
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The assay plate was sealed and incubated at room temperature with gentle shaking for 30 min (300 rpm) while protected from light
