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Renal cortical extracts previously quantified were adjusted to 0.5 g/L, and then 2 L of each diluted extract was incubated with 73 L test solution (0.4 M boric acid, 0.9 M NaCl pH 8.3) with the substrate N-hippuryl-L-histidyl-L-leucine for 25 min at 37C

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Hind Paw Cold Allodynia Test The hind paw cold allodynia test is employed to assess cold thermal sensitivity by assessing the withdrawal response of the rats hind paw when it is delicately immersed in ice-cold water at 4 1 C

L., Davies, M., Van Gaal, L

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