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Introduction Virus-induced gene silencing (VIGS) is a high-throughput reverse genetics technique that exploits an RNA-mediated antiviral defense mechanism [post-transcriptional gene silencing (PTGS)] for functional gene analysis (Ratcliff et al., 1997
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While not all consumers will switch, many are looking for other options as tobacco remains the leading preventable cause of disease and death in the United States, leading to more than 480,000 deaths each year, according to the FDA
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