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Vitamin B12 deficiency in clinical practice
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Purified eIF2 trimers (eIF2) or purified eIF2-P and preassembled eIF2B WT or eIF2B latch decamers were then incubated (with gentle rotation at 4 C) in Assay Buffer (20 mM HEPES-KOH pH 7.5, 150 mM KCl, 5 mM MgCl 2 , 1 mM TCEP, 1 mg ml 1 BSA and 5 mM CaCl 2 ) together for 30 min as indicated
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