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The dried bacterial pellet was solubilized in 20 ml of B-PER (78243, Thermo Fisher Scientific), supplemented with protease inhibitors (1 g ml 1 of leupeptin, 1 g ml 1 of AEBSF HCl and 1 l of benzonase) and 5 mM imidazole and then transferred to a 50-ml centrifuge tube

doi: 10.1007/s00344-021-10570-5 156 WangX.RongW.LiuY.WangX.ZhangZ

These changes may include increasing arterial stiffness, reduced elasticity, impaired function of the blood-vessel lining, chronic inflammation, and the development of atherosclerotic plaque
