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This creates a formidable challenge for researchers seeking reliable and safe compounds for their studies

NAC deacetylates under the action of N-deacetylase to enhance intracellular Cys, thereby increasing the endogenous synthesis of GSH

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a , We constructed a mutant strain that could not synthesize glutathione by knocking out, in the wild-type strain, the GSH1 gene which is essential for glutathione biosynthesis (see Mutant yeasts in the Methods section)