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RNA extraction and library preparation for RNA sequencing Total RNA was isolated from formalin-fixed paraffin-embedded (FFPE) PostNAC surgical specimens ( n = 15) from the same patients whose PreNAC biopsy RNA-sequencing (RNA-seq) data had been analyzed in a previous study [12], enabling direct paired transcriptomic comparisons (Additional File 1)

Human tri peptide GHK-Cu is an anti-aging peptide that is known to enhance collagen production, which is not only beneficial for the skin but also for wound healing and other bodily functions

Personalized dosing respects the fact that one patient's physiology, tolerance, and risk profile may differ from another's