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J Neurosci 31:71747177 Reberger R, DallOglio A, Jung CR et al (2018) Structure and diversity of human dendritic spines evidenced by a new three-dimensional reconstruction procedure for Golgi staining and light microscopy

The homogenate was layered in an ultracentrifuge tube over preformed discontinuous Percoll gradients consisting of a bottom layer of 3 mL of 60% Percoll in buffer B and a top layer of 7 mL of 26% Percoll in buffer B