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PMCID: PMC8653225

2.6 Real-time PCR analysis Total RNA was prepared from liver tissues and LO2 cells using the simple RNA Extract kit (Tiangen Biotech, China) and reverse transcription of RNA to cDNA using Fast King cDNA First Strand Synthesis Kit (Tiangen Biotech, China)

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In three of these studies, penile curvature was also analyzed and was always significantly reduced at the post-treatment controls