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The cells were washed with phosphate buffered saline and collected in 20 mM HEPES (pH 7.4), 150 mM NaCl and 1% NP40
A Skp2 auto-induction loop and restriction point control
Expression of N-terminal His-tagged human GCLM was induced with 1 mM isopropyl--D-thiogalactoside (IPTG, Wako Pure Chemical Industries, Ltd., Osaka, Japan) at 30C for 5 h in Escherichia coli
Bone Basics is unique because it serves not only to reduce bone loss but to maintain or develop bones
10.1039/C4CS00038B Chem
