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Nuclei were counterstained with DAPI (Sigma-Aldrich)

Over time, the elastic fibers become damaged and the collagen network remodels to fit the larger shape

Metabolites were identified by referencing the kyoto encyclopedia of genes and genomes (KEGG), human metabolome database (HMDB), and LipidMaps databases
65,66 Inflammation and long-term ulceration of the colonic mucosa caused by UC may lead to abnormal proliferation and differentiation of colonic mucosal cells, thereby increasing the risk of colorectal cancer
