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Reactions were performed with 50 M RHKKAc, a fluorogenic peptide substrate from p53 residues 379-382, 500 M NAD+ co-substrate, and recombinant human SIRT-1 (NAD+-dependent) enzyme

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These results suggest that transection of the sciatic nerve induces death of the corresponding spinal neurons due to loss of neurotrophic effects, and the overexpression of GDF11 can promote survival of the corresponding neurons