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Finally, 100 L OPD chromogen substrate containing H 2 O 2 were added to each well and the plates incubated, protected from the light, for 15 min and the reaction was stopped by the addition of 50 L 2 M H 2 SO 4

Holotranscobalamin and total transcobalamin in human plasma: determination, determinants, and reference values in healthy adults

10.1016/j.phymed.2021.153526 198 YaoX.LiW.FangD.XiaoC.WuX.LiM.et al (2021)

Cells pre-treated with 20, 40, and 60 M ISO were used as the experimental group

This antihypertensive measure was found to be mediated by nitric oxide
