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Bead pools were resuspended in BSS (500 mM Tris-HCl pH 7.5, 1.5 M NaCl, 50 g/L BSA) and incubated in absence or presence of 10 nM TCEs at 37 C for varying amounts of time, as reported in the figures (typically 24, 8, 4, 2, 1, 0.5, 0.25 h)

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HASMCs (ThermoFisher) were cultured in smooth muscle cell media (ScienCell) containing 2% fetal bovine serum, SMC-growth supplement, and antibiotic-antimycotic

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