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Additionally, histological assessment involved Tyrosine Hydroxylase (TH) staining in the substantia nigra to evaluate the integrity and potential restoration of dopaminergic neurons, with evaluations performed at 1 month and 3 months post-lesion

doi: 10.1016/j.phrs.2018.04.023

Furthermore, microinjections of leptin in rodents, into the ventromedial hypothalamus (VMH) 21 and the arcuate nucleus (ARC) 22 can decrease food intake massively, suggesting that leptins actions are mediated mainly by the hypothalamus

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