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Diluted with Ellman reagent (1:9), the supernatant was taken after 15 min and the absorbance was recorded at 412 nm

Yong WS, Hsu FM, Chen PY (2016) Profiling genome-wide DNA methylation

Aging 129 , 121136 (2023)

So far, three different sources of intake of polyamines in mammals are known: (1) They can be ingested with nutrition;(2) enzymatic synthesis under SAM consumption, hereby the intracellular polyamine concentrations are diet dependent and can be selectively reduced by inhibition of the enzymes required for their biosynthesis [21,22]

However, the macrophages were protected when the thiol, NAC, was used as a pretreatment
