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Institutional animal care and use committee statement: Male Albino Wistar, body weight 200 g, 12 weeks old, randomly assigned, used in all of the experiments, 6 rats/group/interval, approved by local Ethic Committee

Western blotting Protein samples were separated by electrophoresis and transferred to a nitrocellulose membrane (Millipore, USA), blocked with 5% non-fat milk for 2 h, and then incubated overnight at 4 C with primary antibodies specific for PCNA (WANLEIBIO), cyclin A (WANLEIBIO), CDK2 (Bioss), TGF- (Bioss), Caspase 3 (Bioss), Bax (Boster), Bcl-2 (Bioss), MMP2 (Bioss), MMP9 (Bioss), AMPK1 (Bioss), HSP47 (Boster), NLRP3 (WANLEIBIO), Caspase 1 (WANLEIBIO), IL-18 (Affinty), and IL-1 (Bioss)