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To analyze the ubiquitinated products, we diluted the ubiquitination reaction in 2X of loading buffer (Bio Rad) and boiled at 95 C for 5 min
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[DOI] [PMC free article] [PubMed] [Google Scholar] 26.Chiechio S., Zammataro M., Morales M.E., Busceti C.L., Drago F., Gereau R.W., 4th, Copani A., Nicoletti F
