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The hPrdx6 (1 mg/ml) at pH 7.0 and pH 4.0 was subjected to SEC, equilibrated with 3 bed volumes of 10 mM TrisHCl buffer (pH 7.0), and 10 mM sodium acetate buffer (pH 4.0), respectively

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The irony of Koenigs statement is that it captures two of these three primary risks in just two sentences

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Using this compound as a marker of cobalamin deficiency, we have been able to demonstrate that cats and dogs with very low serum cobalamin do indeed have a significant tissue-level cobalamin deficiency (Figure 2)

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