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doi: 10.1016/j.phymed.2019.153132

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Inclusion of these mutations conferred the original Con1 replicon with increased colony formation efficiency, and this phenomenon subsequently was termed adaptive mutation. Specifically, Con1-based replicons carrying two adaptive mutations located at NS3 E176G and T254I, and one at NS5A S225P (Krieger et al., 2001) or NS4B K129T (Lohmann et al., 2003) conferred synergistically enhanced RNA replication

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