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Due to the stochastic nature of the membrane defects that lead to electroporation, the time taken for the membranes to electroporate is variable across replicates

After washing in PBS/FSG, cells were then incubated for 30 min with secondary, anti-mouse IgG antibody conjugated with Nanogold particles (Yaphank, NY, USA) in PBS/FSG (175), washed extensively in PBS/FSG and fixed by incubation with 1% v/v glutaraldehyde in PBS, for 1 h, at room temperature

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Eur J Neurol 30(9):29192945 Akagi M, Mohri I, Iwatani Y, Kagitani-Shimono K, Okinaga T, Sakai N et al (2012) Clinicogenetical features of a Japanese patient with giant axonal neuropathy