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Handle using standard laboratory practices (appropriate PPE, clean tools, and contamination control) in accordance with institutional requirements

Hu Y, Zhou Y, Yang Y et al (2022) Metformin protects against diabetes-induced cognitive dysfunction by inhibiting mitochondrial fission protein DRP1

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Real-Time PCR Total RNA was extracted from cells by acid guanidinium thiocyanate-phenol-chloroform extraction method, and complementary (c)DNA was synthesized using 1 g total RNA in a 20 L RT reaction mix containing 0.5 g/L of random primers, 0.1 mM dNTP, 0.1 M DTT and 5 first strand buffer