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* Molecular weight = 2293, so 1 mm = 229 mg/l

HEK293T cells ( Homo sapiens , female, embryonic kidney cells, ATCC, CRL-1573) were cultured in high-glucose DMEM (Lonza, BE12-604F), 10% FCS (Biochrome, S0115), HEPES (10 mM, Biochrome, L1613), non-essential amino acids (1 mM, Gibco, 11140-035), 100U/ml penicillin and 100 g/ml streptomycin (1% Biochrome, 0257 F) and 2 mM sodium pyruvate (1% Biochrome, L 0473)

It represents a promising first-line treatment for DPN, preventing early development and progression through both direct and indirect antioxidant effects [8,23,71,87,110] (Figure 3)

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More recently, a novel N-terminal TAP tag denominated as CHiC tag was constructed based on a combination between His-tag and a choline binding domain followed by a cleavage site specific for TEV protease with applications on protein purification (Stamss et al., 2013)