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Explants were grown in phenol-red Neurobasal media (Invitrogen) supplemented with 2% B27 serum-free supplement (Invitrogen), 1% l-glutamine (Wisent), 1% penicillin/streptomycin (Wisent), 10 m 5-fluoro-2-deoxyuridine (FDU

These results all suggest that ES2 has minimal toxicity after multiple doses in aged mice

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MitoTEMPOs targeted action protects mitochondrial function and reduces lipid ROS formation, safeguarding both alveolar epithelial and endothelial cells from ferroptotic cell death (97)