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Washing the column with 100 m1 equilibration buffer and elution of the adsorbed proteins was achieved with 10 mM GSH contained in 0.05 M TrisHCl buffer pH 8.0

Symptoms include thirst, frequent urination, weight loss and an increased susceptibility to infection
Metabolomics Workbench: an international repository for metabolomics data and metadata, metabolite standards, protocols, tutorials and training, and analysis tools
We also extracted data pertaining to the number of intrauterine gestational sacs and fetal heart rate visible by transvaginal ultrasound within 12 weeks of intervention (clinical pregnancy rate), the number of visible leading follicles of more than or equal to 18 mm by transvaginal ultrasound within 12 weeks of intervention (ovulation rate), BMI in kg/m2, serum LDL, serum HDL, TG, total cholesterol in mmol/l or mg/dl, and fasting blood glucose (FPG) in mg/dl, serum FSH and LH in IU/L, mental health status assessment using any questionnaires, and adverse side effects such as gastrointestinal disturbances (abdominal pain, nausea, and vomiting

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