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The product is used for elongation with two further carbons by reaction with another malonyl-ACP at step 3

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Younger researchers often see subtle effects because their baseline GHK levels remain relatively high

The absorbance data produced can be compared to a standard curve to determine the initial reaction velocity (production formation rate) and used to construct a Lineweaver-Burk Plot to calculate the Km and Vmax values for each respective enzyme reaction

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