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doi:10.3390/ph17081081

243,244 Nucleobase modification Nucleobases are essential components of nucleic acids, and changes to their structure can affect the stability, biological activity, and immunogenicity of nucleic acids

Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles

0.52.5 min, 95% B