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Similarly, for overexpression cell lines, gBlocks (IDT) containing the cDNA of interest were cloned into pMXS-IRES-Blast linearized with BamHI and NotI by Gibson Assembly (NEB)

p21 exerts this anti-apoptotic function through several key mechanisms, predominantly when localized in the cytoplasm

More precisely, the complex bearing the most lipophilic benzoate ligand, 3P , showed the highest in vitro antiproliferative activity (mean IC 50 equal to 2.6 M), whereas the complexes with a more hydrophilic carboxylate, such as succinate and acetate, 4P and 5P , were endowed with a lower cytotoxic activity (mean IC 50 of 30.4 and 11.1 M, respectively)

Airway epithelial cell-specific delivery of lipid nanoparticles loading siRNA for asthma treatment

[DOI] [PubMed] [Google Scholar] 101.Wang L.A., de Kloet A.D., Smeltzer M.D., Cahill K.M., Hiller H., Bruce E.B., Pioquinto D.J., Ludin J.A., Katovich M.J., Raizada M.K., Krause E.G