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This process promotes LPO, leading to ferroptosis owing to ROS produced by mitochondria

Prusakiewicz JJ, Duggan KC, Rouzer CA, Marnett LJ (2009) Differential sensitivity and mechanism of inhibition of COX-2 oxygenation of arachidonic acid and 2-arachidonoylglycerol by ibuprofen and mefenamic acid

The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mmol/L butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, United States) and sonicated for 30 s in an ice bath (Ultrasonic bath, Branson, United States)

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Studies from the Butterfield laboratory reported the increased oxidation of several enzymes involved in glucose catabolism, as follows: fructose bisphosphate aldolase (FBA), triose-phosphate isomerase (TPI), phosphoglucomutase 1 (PGM1), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), alpha-enolase (Eno1), and pyruvate kinase (PK) (TABLE 4 and FIGURE 10)