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Briefly, plasma samples were separately incubated with renin substrate angiotensinogen at 37 C and 0 C (pH = 6.0) for 2 h to generate angiotensin enzyme I (Ang-I)

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A 2-week baseline period was established with control flour before the households were randomly separated into two groups for the purposes of the study (Figure 1), one which received Zincol-2016/NR-421 grain for 8 weeks and then Galaxy-2013 control grain for 8 weeks (study arm 1 (SA1)), whilst the other received Galaxy-2013 control grain for 8 weeks followed by Zincol-2016/NR-421 grain (study arm 2 (SA2)) (Lowe et al., 2018)

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