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Synthesis of peptides in enzymatic reactions involving glutathione

All assays were previously checked for specificity (melting curve and agarose gel electrophoresis of PCR products) and reaction efficiency, dynamic range and linearity (standard curve experiments) and reproducibility (estimation of the coefficient of variation-CV by analysing a series of samples in different runs)

Anti-Aging: This treatment can help reduce the signs of aging by promoting collagen production, resulting in firmer, more youthful-looking skin

Each approach has genuine trade-offs

4c,d), which is consistent with their close evolutionary relationship (Fig