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Notably, some research performed using NPs such as gold and silver on mature spermatozoa have reportedly contributed to increased loss of mobility with a consequent limitation on the fertilizing power of human spermatozoa [119] and bovine sperm cells [120]

(4) Using the Perkin Elmer Filtermate Harvester to filter the reaction mixture through the GF/C plate, then wash each plate four times with cold wash buffer

doi: 10.1038/nrneph.2015.82 241 Rubio-NavarroASanchez-NioMDGuerrero-HueMGarca-CaballeroCGutirrezEYusteCet al

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(107) demonstrated that even though prednisolone increased serum total and bioactive IGF-I, post-IGF-IR signaling was inhibited indicating that prednisolone-induced increases in serum IGF-I levels is not accompanied by increased IGF-I action