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The cell precipitate was resuspended in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco, 15640055), and the cells were seeded

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2b), which we conclude is the causal variant for this association (Supplementary Table 13)

Here we show that SIRT3, a mitochondrial deacetylase highly expressed in HSCs but reduced during aging, suppresses the HSC response to aging that drives maladaptive trained immunity, chronic inflammation and tissue functional decline in mice