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Capsaicin (CAP), a natural active ingredient in Capsicum annuum L
Free Radicals in Biology and Medicine, Chapter 2

Cystathionine is subsequently cleaved by cystathionine -lyase (also called cystathionase) to produce cysteine and -ketobutyrate (also referred to as 2-ketobutyrate or 2-oxobutyrate)

As a standard curve to calculate thiol content, DTT powder (Gold Biotechnology, DTT50) was freshly dissolved in 0.4 M sodium citrate, pH 4.5, 1 mM EDTA, 5% SDS and a dilution series from 0 to 3 nmol was prepared and assayed in parallel with samples
