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Biochemical measurements At the end of the reperfusion period, animals were euthanized with a high-dose intraperitoneal injection of ketamine and xylazine (200 mg/kg and 20 mg/kg, respectively), and ileal tissues were harvested and stored at 80 C for biochemical analysis

Inflammatory response can lead to tissue oxidative stress, cell apoptosis and fibrosis

Vitamin D may have antithrombotic effects by reducing cytokine-induced changes in tissue factor and thrombomodulin, as suggested by several experimental studies (Ueno et al., 2019)