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Description
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GSH conjugation to the enolised ketone intermediates of ecosanoids could protect cells from their toxic actions

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Biliary tree fragments and associated stroma were dissociated into single cells using 7x TrypLE (Gibco), incubated in 2% FCS with antibodies anti-EpCAM/CD326 (clone G8.8, APC, eBioscience, 6:100), CD45 (clone 30-F11, PE, BioLegend, 1:50), CD31 (clone 390, PE, BioLegend, 1:50), TER-119 (clone TER-119, PE, BioLegend, 1:50) and subjected to fluorescence-activated cell sorting (FACS) based on size and granularity and singlets using a BD Influx Cell Sorter

These studies collectively contribute new insights into understanding the role of oxidative stress in IBD and potential therapeutic avenues targeting ROS-mediated pathways in the management of this condition
