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Research is investigating biomarkers that could predict responsiveness to NAD+ enhancement, optimal dosing strategies for different applications, and combination approaches that pair NAD+ precursors with other interventions for synergistic effects

Thin sections were cut using an ultramicrotome (Leica Microsystems, Germany), and the sections were subjected to double staining with uranyl acetate and lead citrate and then observed using a transmission electron microscope (Hitachi HT7800, Japan)

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Gene Expression